The metabolism of pyruvate in bull spermatozoa.

Effect of the Addition of Insulin Like Growth Factor-I (IGF-I) on Cryopreservation of Hariana Bull Semen

This experiment was designed to study the effect of insulin like growth factor-I (IGF-I) on cryopreservation of Hariana bull semen. For this purpose, semen ejaculates were collected from four Hariana bulls using artificial vagina at biweekly interval. The semen samples which possess more than 70% initial progressive motility and above 500 million/mL spermatozoa concentration was subsequently su...

Spermatozoa Molecules in Relation to Bulls Fertility

Bull fertility may be defined as the process by which spermatozoa fertilize and activate the ovum and then support embryonic development. Bull fertility is a complex trait having relatively low heritability and plays a vital role for efficient production and reproduction of bovine. Various mechanisms involved in regulating bull fertility associated phenotype and reliable biomarkers are poorly d...

O-12: Studies on Sequestration of PDC-109 Protein on Cryodamage and In Vitro Fertility of Crossbred Bull Spermatozoa

Background: Plasma membrane of the spermatozoa interacts with and is altered by abundant seminal PDC-109 present in their immediate milieu in vivo with damaging effect in a time- and concentration- dependent manner. Therefore we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial for cryopreservation of sperm cells. To this aim we evaluated the effect of s...

RNA extraction and leptin receptor mRNA detection in bull ejaculated spermatozoa

The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa

The aim of this study was to determine the effects of the antioxidants superoxide dismutase (SOD),glutathione peroxidase (GPx) and butylated hydroxytoluene (BHT) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. Ejaculates were collected from five Holsteinbulls and pooled at 37°C. The semen samples were diluted with a CEY extender containing addit...

P-116: The Catalase Effect on LDH Enzyme Leakage of Buffalo Epididymal Sperm During Culture in Human Tubal Fluid Mediaa

Background: Mammalian spermatozoa are highly susceptible to lipid peroxidation, which leads to structural damage to the sperm cell, the enzymatic leakage from spermatozoa accompanied by lowered motility and metabolism. Catalase, an enzyme that detoxify hydrogen peroxide and reduce the enzyme leakage from sperm cell Materials and Methods: For this study, buffalo bull testicles (20 pairs) were pi...